Acellular porcine aorta matrix as a novel tissue engineered vascular scaffold / 中国组织工程研究

BACKGROUND: The key of tissue engineered blood vessel research depends on the appropriate scaffolds. The porcine vascular tubes have frequently been used as candidates for tissue engineering vessel construction, but high immunogenicity and poor mechanical strength limit its application for tissue engineering scaffolds.OBJECTIVE: To prepare a novel tissue engineered vascular scaffold with good mechanical properties and biocompatibility using acellular porcine aorta matrix.METHODS: Porcine aorta was decellularized and modified by thermal cross-linking to improve the mechanical strength and biodegradation properties and to prepare acellular porcine aorta matrix scaffolds. Hematoxylin-eosin staining of histological sections and biomechanical tests were performed to assess the decellularization effects and the mechanical strength of the vascular matrix respectively. Vascular endothelial cells from human umbilical cord veins were isolated and seeded on the acellular matrix scaffolds and cultured in vitro. The biocompatibility was evaluated. RESULTS AND CONCLUSION: After the porcine aorta was treated by 1% Triton X-100 solution for 84 hours, the vessel was fully decellularized, and the architecture of matrix was well preserved. The acellular vascular matrix demonstrated improved biomechanical properties after modification by thermal cross-linking under vacuum at 120 °C for 12 hours. Its tensile strength reached 1.70 MPa. After 7 days of in vitro culture, the seeded endothelial cells formed a typical vascular endothelial layer structure on the surface of acellular matrix, as observed by scanning electron microscopy. Results proved that acellular vascular matrix of porcine aorta could maintain the morphology and structure of natural vessels, its mechanical strength could be greatly improved after successive freeze-drying and thermal cross-linking. A good compatibility between the acellular matrix and endothelial cells of umbilical vein is also achieved.

Prevention of surgical complications of renal transplantation operation / 中国基层医药

Objective To investigate the potential complications of renal transplant patients, to develop appropriate treatment programs to reduce the incidence. Methods Clinical data of 42 patients with renal transplantation were retrospectively analyzed. Blood type of the donors and recipients were same in 32 cases; Blood type of the donors was 0 and recipients blood type was A,3 cases;Donors blood type was 0 and recipients blood type was B,2 cases; Donors blood type was O and recipients blood type was AB,4 cases; Donors blood type was B and recipients blood type was AB,lease. Results In alll cases, there were acute rejection in 2 cases,pulmonary infection in 2 cases, wound infection in 1 case,urinary leakage in 1 case,urinary tract infection in 2 cases.42 cases were all cured up. One patient lost kidney function and required dialysis therapy 4 years after kidney transplantation, the remaining 40 patients were good. Conclusion Early detection and timely management of complications of renal transplantation played a very important role in improving the survival rate.

The imitation release test of extra-body on sustained-released injection of microcapsule hydro-cortisone / 中国基层医药

Objective By extra-body imitation release test with prolongation,medicines action on sustainedreleased injection of hydro-cortisone microeapsule was understood. Methods Making use of HPLC method was inspected to result of medicinal lower and die, solution. At the same time, the disaolvent course of microcapsule wall was observed by microscope and electron microscope. Results The release test at beginning 12 - 24 hours with rdease drugs measure was very lower-degree,along with the time more prolong in mierocapsule substance to contact of PBS solution(24～48h) was began disintegrate. The rdease of medicinal concentration also accompany with rising, the rising different concentration PBS solution from starting up to the highest as to general need 3～5 days and keep releasing for several days. Conclusion Sustained-released injection of hydrocortisone mierocapsule can be effectual action of prolongation released time in hind hydrocortisone.

Current researches in keratoprosthesis / 生物医学工程学杂志

This paper introduced the evolution of keratoprosthesis (KPs) from the earliest devices to the newly developed types, pointed out their drawbacks and discussed the properties that an ideal keratoprosthesis or a tissue engineering keratoprosthesis must possess. Recent researches focused on the use of porous polymers as the skirt of core-skirt keratoprosthesis and tried to improve the material's biologicial intergration.

Study of the Biocompatibility Between Bone Morphogenetic Protein Microspheres and Bone Marrow Stem Cell / 中国医师杂志

Objective To evaluate the biocompatibility between bone morphogenetic protein(BMP) composition and bone marrow stem cell (BMSc).Methods Microspheres were prepared with chitosan and sodium alginate, and BMP was enwrapped in the microspheres. The biocompatibility of the composition was examined using cell-culturing method. The BMSc was cultured in combination with microspheres. The extending speed of the cells, the proliferation and alkaline phosphatase activity were tested.Results There were no inhibition on cellular proliferation of BMSc when it was cultured in combination with microspheres in vitro, but ALP activity increased significantly.Conclusion BMP microspheres possessed satisfactory biocompatibility, and could increase the osteogenic capability of BMSc in vitro.

Reconstruction of tissue engineering blood vessel via acellular matrix / 中国病理生理杂志

AIM:To reconstruct the tissue engineering blood vessel by using acellular matrix of porcine thoracic aorta tissue as the scaffold, and by inoculability of vascular endothelial cells (EC) from human umbilical cord vein. METHODS: The porcine thoracic aorta was treated with 1% Triton X-100 for preparing acellular vessel matrix, and the mechanical characterization was in succession modified by freeze-drying and thermal cross-linking techniques. Meanwhile, the mechanics capability of the vessel was measured. The endothelial cells isolated from umbilical cord vein were seeded on the acellular matrix scaffolds by tissue culture in vitro. The structure of acellular matrix was analyzed by light microscopy and scanning electron microscopy. RESULTS: By treatment with 1% Triton X-100 for 84 h, the cells of thoracic aorta were fully come off and the three-dimensional structure of the matrix still remained. After modification by freeze-drying for 24 h and then thermal cross-linking under vacuum at 120 ℃ for 12 h, the tensile strength of the acellular matrix remarkable increased and reached the maximum breaking strength of 1.70 MPa. It was also showed that cultured endothelial cells grew on the surface of acellular matrix for 7 days and the typical structure of vessel-like intimal formation was observed under scanning electron microscopy. CONCLUSION: The acellular matrix and endothelial cells have favorable compatibility. The modified acellular matrix could be a good candidate scaffold for rebuilding the tissue engineering blood vessel.